The BBC will broadcast the FA Cup fourth qualifying round tie between Dunston UTS and Gateshead.
Dunston, FA Vase winners in 2012, are the lowest-ranked club left in the competition and face National League opponents in a North East derby for a place in the first round proper.
The tie will be streamed live on the BBC Sport website and app and Connected TV from 12:20 BST on 20 October.
As well as progressing, the winners will receive £25,000 in prize money.
Dunston are seventh in Division One of the Northern League – four levels below Gateshead – but have as many as six games in hand on the teams above them in the league.
They have already beaten Hebburn Town 3-2, Pontefract Collieries 4-2, North Ferriby United 4-1, Irlam 2-1 and Chester FC 4-3 to reach this stage.
Gateshead, eighth in the National League, reached the second round of the FA Cup last season, when they lost 5-0 to Luton Town, and the third round in 2014-15, losing 7-0 to West Brom.